DeMaRes Study

Prevalence of macrolide and fluoroquinolone resistance-mediating mutations in Mycoplasma genitalium and Mycoplasma pneumoniae

Posted on 08 April 2021 Views: 0 Go to project: 0
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Organizers

  • Interregional Association for Clinical Microbiology and Antimicrobial Chemotherapy (IACMAC)
  • Institute of Antimicrobial Chemotherapy (IAC), Smolensk State Medical University

Annotation

Mycoplasma genitalium is a common sexually transmitted pathogen in men and women.

Mycoplasma pneumoniae is an ‘atypical’ bacterial pathogen that causes lower respiratory infections, including pneumonia and tracheobronchitis, and less commonly, upper respiratory tract infections.

This dataset presents the results of longitudinal surveillance study of mutational resistance to macrolides (mutations in domain V of the 23S rRNA gene) and fluoroquinolones (mutations in quinolone resistance-determining regions (QRDRs) of gyrA and parC genes) of M. genitalium and M. pneumoniae.

Study protocol

Sources of clinical samples

M. genitalium PCR-positive urogenital samples (n=873) were collected in five regions of Russia: Moscow, Nizhny Novgorod, Saratov, Smolensk, and Tula, in 2009-2019.

M. pneumoniae PCR-positive samples (n=1480) were collected in twelve regions of Russia: Bryansk, Khabarovsk, Krasnoyarsk, Mari El Republic, Moscow, Moscow Region, Nizhny Novgorod, Penza, Smolensk, Sverdlovsk, Tula, and Yaroslavl, in 2006-2020.

All samples were collected as part of routine diagnostics for genitourinary and respiratory infections and were non-duplicate (represented individual patients/cases of infection). PCR-positive samples were referred to the IAC central laboratory for analysis of antibiotic resistance-associated mutations

Detection of macrolide and fluoroquinolone resistance-associated mutations

Single nucleotide substitutions at positions 2058-2062 and 2611 of domain V of the 23S rRNA and mutations conferring amino acid (aa) changes at QRDR regions of ParC (aa positions 79-84) and GyrA (aa positions 83-87) were detected by means of probe-based real-time PCR assays and were confirmed by independent PCR amplification and Sanger sequencing of the corresponding gene fragments [2-4].

The data on the types of mutations detected is presented under the ‘Markers’ section of the website.

Study group:

IAC laboratory:

  • Edelstein I.A.,
  • Romanov A.V.,
  • Edelstein M.V.,
  • Kuzmenkov A.Yu.,
  • Kozlov R.S.

Participating centers:

  • Aksyutina G.V. (Bryansk)
  • Alyabyeva N.A. (Moscow)
  • Borisov I.V. (Saratov)
  • Groshenkova E.V. (Yaroslavl)
  • Gushin A.E. (Moscow)
  • Ershova M.G. (Yaroslavl)
  • Enikeeva F.S. (Penza)
  • Zaitsev A.A. (Moscow)
  • Zubareva L.M. (Smolensk)
  • Ignatkova A.S. (Tula)
  • Ivanova O.V. (Smolensk)
  • Ivanova I.A. (Yoshkar-Ola)
  • Kolesnikova E.A. (Nizhny Novgorod)
  • Majarova O.A. (Minsk, Belarus)
  • Novikova O.P. (Tula)
  • Olenkova O.M. (Ekaterinburg)
  • Pleskachevskaya T.A. (Smolensk)
  • Protasova I.N. (Krasnoyarsk)
  • Rudneva N.S. (Tula)
  • Semenova O.L. (Bryansk)
  • Tsykhanovskaya O.A. (Tver)
  • Yatsyshina S.B. (Moscow)

Coordinator

Inna Edelstein, PhD

Head, Laboratory of Molecular Diagnostics, IAC

inna.edelstein@antibiotic.ru

НИИ антимикробной химиотерапии ФГБОУ ВО «СГМУ» Минздрава России

Publications

  1. Edelstein I.A. Mycoplasma pneumoniae – modern data on the structure, molecular biology and epidemiology of the pathogen. Clinical Microbiology and Antimicrobial Chemotherapy. 2023; 25(4):332-349 DOI: 10.36488/cmac.2023.4.332-349
  2. Edelstein I.A., Guschin A.E., Romanov A.V., Negasheva E.S., Kozlov R.S. Genetic Determinants of Macrolide and Fluoroquinolone Resistance in Mycoplasma genitalium and Their Prevalence in Moscow, Russia. Pathogens. 2023; 12(3):496. DOI: 10.3390/pathogens12030496
  3. Edelstein I.A., Romanov A.V., Kozlov R.S. Development of a Real-Time PCR Assay for Detection of Macrolide Resistance Mutations in Mycoplasma genitalium and Its Application for Epidemiological Surveillance in Russia. Microbial Drug Resistance.Mar 2023.69-77. DOI: 10.1089/mdr.2022.0131
  4. Edelstein, I.A.; Ivanova, O.V.; Romashov, O.I.; Kozlov, R.S. Course of Lower Respiratory Tract Infection in Young People Treated at the Military Hospital of Smolensk Garrison with Detected Mycoplasma pneumoniae Carrying a Macrolide-Resistant Mutation in 23S rRNA Gene. Pathogens 2023, 12, 103. DOI: 10.3390/pathogens12010103
  5. Edelstein I.A., Rudneva N.S., Romanov A.V., Zubareva L.M., Kuzmenkov A.Yu., Kolesnikova E.A., Trushin I.V., Borisov I.V., Sukhanova L.N., Akhmedova A.M., Novikova O.P., Kozlov R.S. Monitoring of macrolide resistanceassociated mutations in Mycoplasma genitalium in Russia. Clinical Microbiology and Antimicrobial Chemotherapy. 2022; 24(1):52-60. DOI: 10.36488/cmac.2022.1.52-60
  6. Romanov A.V., Kozlov R.S., Edelstein I.A., Edelstein M.V. Method of detection of fluoroquinolone resistance-associated mutations genes parC and gyrA QRDR in Mycoplasma genitalium. Patent # RU 2778666 C1, 22.08.2022. Ref.
  7. Ivanova O.V., Edelstein I.A., Romashov O.I., Kozlov R.S. Effect of 23S rRNA gene mutations in Mycoplasma pneumoniae on severity of community acquired pneumonia in young adult patients treated at the Smolensk military hospital. Clinical Microbiology and Antimicrobial Chemotherapy. 2020; 22(4):306-312. DOI: 10.36488/cmac.2020.4.306-312.
  8. Zubareva L.M., Edelstein I.A., Rudneva N.S., Romanov A.V., Vlasova T.A., Lavrinenkova Yu.V., Sukhanova L.N., Ahmedova A.M., Kuzmenkov A.Yu., Trushin I.V., Evstafev V.V. The rates of mutations associated with macrolide resistance in Mycoplasma genitalium among patients with non-gonococcal sexually transmitted infections in Smolensk and Tula. Clinical Microbiology and Antimicrobial Chemotherapy. 2019; 21(4):330-339. DOI: 10.36488/cmac.2019.4.330-339
  9. Zubareva L.M., Edelstein I.A., Romanov A.V., Evstafev V.V., Kozlov R.S. Clinical case of failure of josamycin in a patient with urethritis caused by Mycoplasma genitalium. Vestnik Dermatologii i Venerologii. 2018;94(4):55-59. DOI: 10.25208/0042-4609-2018-94-4-55-59
  10. ​Edelstein I.A., Edelstein M.V., Romanov A.V., Zaitsev A.A., Rakovskaya I.V., Barkhatova O.I., Antipushina D.N., Kozlov R.S. Four cases of resistance mutations in 23S rRNA gene in Mycoplasma pneumoniae isolated from the hospitalized military personnel. Clinical Microbiology and Antimicrobial Chemotherapy. 2017; 19(3):248-253. Ref.
  11. Romanov A.V., Kozlov R.S., Edelstein I.A., Edelstein M.V. Method of detection of macrolide resistance-associated mutations in Mycoplasma genitalium and Mycoplasma pneumoniae. Patent # RU 2646123 C1, 01.03.2018. Ref.
  12. Edelstein I.A., Edelstein M.V., Romanov A.V., Ratchina S.A., Yatsyshina S.B., Rakovskaja I.V., Kozlov R.S. Detection of macrolide-resistance mutations in 23S rRNA gene of Mycoplasma pneumoniae using a novel real-time PCR assay. Pacific Medical Journal. 2015; (1):63-66. Ref.
  13. Shipitsyna E, Rumyantseva T, Golparian D, Khayrullina G, Lagos AC, Edelstein I, Joers K, Jensen JS, Savicheva A, Rudneva N, Sukhanova L, Kozlov R, Guschin A, Unemo M. Prevalence of macrolide and fluoroquinolone resistance-mediating mutations in Mycoplasma genitalium in five cities in Russia and Estonia. PLoS One. 2017. Apr 13; 12(4). DOI: 1371/journal.pone.0175763
  14. Edelstein I., Rachina S., Touati A., Kozlov R., Henin N., Bébéar C., Pereyre S. Mycoplasma pneumoniae Monoclonal P1 Type 2c, Outbreak, Russia, 2013. Emerging Infectious Diseases. Vol. 22, No. 2, February 2016. DOI: 10.3201/eid2202.151349
  15. Bobylev A.A., Rachina S.A., Eidelshtein I.A., Kozlov R.S., German S.V., Pogodin A.G. Outbreak of Mycoplasma pneumoniae infection at Smolensk region. PULMONOLOGIYA. 2013;(5):97-100. (In Russ.) Ref